bamsifter

Normalizes coverage depth by position-specific read sampling in a bam file to give a target max depth

Usage:

bamsifter [-c max_coverage] [-i max_identical_cigar_pos] [-o out.bam] [--FLAGS] <in.bam>

Options:

-c                    Max coverage value
-o                    Output file name. Default is stdout
-i                    Max number of reads with an identical cigar starting at the some position to keep
--keep_unmapped       Keep unmapped reads (0x4 flag)
--keep_secondary      Keep alignments flagged as secondary (0x100 flag)
--keep_supplementary  Keep alignments flagged as supplementary (0x800 flag)
--keep_chimeric       Keep chimeric alignments (SA: tag)

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engineered by Christophe Georgescu @ BroadInst