scRNA_Tutorial

NOTE : This single-cell tutorial is dervied from different sources and I would like to thank the individuals maintaining the resources and making them available for wider research community. The sources for each tutorial are acknowledged in each individual tutorial section.

Tutorial Layout

The tutorial covers Data download from SRA and cellranger preprocessing to generate count matrix. The count matrix then can be imported in Seurat to create a Seurat object. Besides the data download and Cellranger tutorial covers three aspects of single cell analysis

• Generating count Matrix: Data download and Cellranger (fastq to count matrix)
• Single Sample scRNA analysis.
  • ScRNA Quality check
  • scRNA Cellcycle stage evaluation and analysis
• Case-Control scRNA sample analysis
• Trajectory analysis

The markdown file for each section of the tutorial is in each folder.