integration of RE Galaxy

files/galaxy-re.grid.cesnet.cz/README

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+# usegalaxy
+
+Simple galaxy installation steps.
+
+On fresh install of ubuntu:
+```
+sudo apt-get update
+sudo apt-get upgrade -y
+sudo apt update
+sudo apt upgrade -y
+sudo apt install -y software-properties-common
+sudo apt-add-repository --yes --update ppa:ansible/ansible
+sudo apt install -y ansible
+```
+
+Clone the repo and instal required galaxy roles.
+```
+git clone https://github.com/CESNET/usegalaxy.git
+cd usegalaxy
+ansible-galaxy install -p roles -r requirements.yml
+```
+Note that the roles are already present in this repo.
+
+After the instalation, you should configure your [inventory file](https://training.galaxyproject.org/training-material/topics/admin/tutorials/ansible/tutorial.html#inventory-file) ([hosts](https://github.com/CESNET/usegalaxy/blob/main/hosts)) and set up a vault:
+```
+openssl rand -base64 24 > .vault-password.txt
+ansible-vault create group_vars/secret.yml
+```
+The second command opens an editor wherein you should define variables eg.:
+```
+vault_id_secret: "a-long-random-value"
+rabbitmq_users_password:
+  mqadmin: "a-long-random-value"
+  galaxy: "a-long-random-value"
+  galaxy_gpu: "a-long-random-value"
+```
+The value should be a long random value, which can be obtained by command `openssl rand -base64 24`, though you should avoid having a `/` or `\` in your password as galaxy will interpret this as a path.
+
+Make sure you have these ports enabled on your machine:
+```
+80(HTTP)
+443(HTTPS)
+4369(TCP)
+5671(TCP)
+15672(TCP)
+25672(TCP)
+```
+
+Once everything have been installed and configured, the ansible playbook can be run as follows:
+```
+ansible-playbook galaxy.yml
+```
+
+## Usegalaxy tools
+
+Install ephemeris for tool management:
+```
+virtualenv -p python3 ~/ephemeris_venv
+. ~/ephemeris_venv/bin/activate
+pip install ephemeris
+```
+To obtain the list of tools from the [usegalaxy.eu](https://usegalaxy.eu) use:
+```
+get-tool-list -g "https://usegalaxy.eu" -o "eu_tool_list.yaml"
+```
+and then to install them:
+```
+shed-tools install -g https://your-galaxy -a <api-key> -t eu_tool_list.yaml
+```
+The api key can be found in the User -> Preferences -> Manage API Key in Galaxy menu.
+
+...
+
+Later we should use this [role](https://github.com/galaxyproject/ansible-galaxy-tools) to automate this, but there are too many tools to be installed and it is very inpractical for testing purposes. 
+
+## Debian 11
+
+### TUS
+
+On ubuntu < 22.04 and Debian < 12 the GLIBC version is incompatible with the ```galaxyproject.tusd``` GLIBC, therfore playbook ends in error.
+- in `roles/galaxyproject.tusd/defaults/main.yml` you have to set the `tusd_version:` to 'v1.8.0' (tested, possibly higher)
+
+### Ansible
+
+On Debian 11 the default version of `ansible` is 2.10.08 which fails to extract binaries for TUSd.
+- workaround:
+  - because of Puppet, we need to create dedicated sources.list file for ansible
+  - `sudo vim /etc/apt/sources.list.d/ansible_ubuntu_focal_main.list` add this line : `deb http://ppa.launchpad.net/ansible/ansible/ubuntu focal main`
+  - then `sudo apt-key adv --keyserver keyserver.ubuntu.com --recv-keys 93C4A3FD7BB9C367`
+  - followed by `sudo apt-get update` and `sudo apt-get install ansible -y`
+- Note that you might need to `sudo apt remove ansible` `sudo apt autoremove` before you try to install ansible.
+
+### Nginx Upload Module
+
+Our playbook makes use of `nginx-upload-module`, which is not included by default in any nginx-* distribution from `apt`.
+This is a simple recipe for installing nginx with the upload-module.
+
+```
+sudo apt remove *nginx* -y &&
+sudo apt autoremove -y &&
+
+wget http://nginx.org/download/nginx-1.24.0.tar.gz &&
+tar -xzf nginx-1.24.0.tar.gz &&
+cd nginx-1.24.0/ &&
+git clone https://github.com/hongzhidao/nginx-upload-module.git &&
+sudo apt-get install -y libpcre3 libpcre3-dev libssl-dev zlib1g-dev &&
+./configure --user=nginx --group=nginx --prefix=/etc/nginx --conf-path=/etc/nginx/nginx.conf --sbin-path=/usr/sbin/nginx --http-log-path=/var/log/nginx/access.log --error-log-path=/var/log/nginx/error.log --lock-path=/var/lock/nginx.lock --pid-path=/run/nginx.pid --modules-path=/usr/lib/nginx/modules --http-client-body-temp-path=/var/lib/nginx/body --http-fastcgi-temp-path=/var/lib/nginx/fastcgi --http-proxy-temp-path=/var/lib/nginx/proxy --http-scgi-temp-path=/var/lib/nginx/scgi --http-uwsgi-temp-path=/var/lib/nginx/uwsgi --with-compat --with-debug --with-pcre-jit --with-http_ssl_module --with-http_stub_status_module --with-http_realip_module --with-http_auth_request_module --with-http_v2_module --with-http_dav_module --with-http_slice_module --with-threads --with-http_addition_module --with-http_gunzip_module --with-http_gzip_static_module --with-http_sub_module --add-module=nginx-upload-module &&
+make && make install
+```
+
+Note that you might need to `sudo systemctl unmask nginx.service` and remove the `galaxy` and `redirect-ssl` from `/etc/nginx/sites-available and `/etc/nginx/sites-enabled` before running the playbook again.
+
+### Other issues
+
+
+Use `sudo apt install python3-docker` before running the `usegalaxy.rabbitmqserver` role.
+
+For influxdb apt key error: https://www.influxdata.com/blog/linux-package-signing-key-rotation/
+
+For proftpd add "LoadModule mod_tls.c" to roles/galaxyproject.proftpd/templates/tls.conf.j2 just before the TLSEngine 

files/galaxy-re.grid.cesnet.cz/all_tools.yml

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+# DEMON: This file is a copy from usegalaxy.cz, it wasn't adjusted to the needs of RE yet
+name: Tools
+type: generic
+id: all_tools
+items:
+- sections:
+  - upload_file
+  - get_data
+  - data_source_tools
+  - send_data
+  - collection_operations
+  - expression_tools
+- id: general_text_label
+  text: General Text Tools
+  type: label
+- sections:
+  - text_manipulation
+  - filter_and_sort
+  - join__subtract_and_group
+  - convert
+  - convert_formats
+  - builtin_converters
+- id: genomic_manip_label
+  text: Genomic File Manipulation
+  type: label
+- sections:
+  - fasta_fastq
+  - fastq_quality_control
+  - quality_control
+  - sam_bam
+  - bed
+  - vcf_bcf
+  - nanopore
+  - lift_over
+  - extract_features
+  - operate_on_genomic_intervals
+  - fetch_sequences___alignments
+  - chromosome_conformation
+- id: genomics_analysis_label
+  text: Genomics Analysis
+  type: label
+- sections:
+  - assembly
+  - annotation
+  - mapping
+  - variant_calling
+  - rna_analysis
+  - multiple_alignments
+  - hgv
+  - evolution
+  - regional_variation
+  - biodiversity_data_exploration
+  - genome_diversity
+  - rna_seq
+  - peak_calling
+  - genome_editing
+  - epigenetics
+  - phylogenetics
+  - phenotype_association
+  - single_cell
+  - hca_single_cell
+  - hca-single_cell
+  - single-cell
+- id: stats_viz
+  text: Statistics and Visualization
+  type: label
+- sections:
+  - statistics
+  - machine_learning
+  - plots
+  - interactive_tools
+  - graph_display_data
+- id: genomics_toolkits_label
+  text: Genomics Toolkits
+  type: label
+- sections:
+  - mothur
+  - qiime
+  - qiime_2
+  - picard
+  - deeptools
+  - emboss
+  - ncbi_blast
+  - mimodd
+  - scanpy
+  - hicexplorer
+  - gemini
+  - motif_tools
+  - gatk_tools
+  - rad_seq
+  - sanger_sequencing
+  - dna_metabarcoding
+  - apollo
+- id: domain_tools_label
+  text: Domain Tools
+  type: label
+- sections:
+  - virology
+  - metagenomic_analysis
+  - proteomics
+  - metabolomics
+  - muon_spectroscopy
+  - chemicaltoolbox
+  - astronomy
+  - imaging
+  - climate_analysis
+  - gis_data_handling
+  - spatial_omics
+  - ecoregionalization
+  - graphclust
+  - species_abundance
+  - data_and_metadata_management
+  - obo_ontology_manipulation
+  - ontology
+  - compute_indicators_for_satellite_remote_sensing
+  - compute_indicators_for_turnover_boulders_fields
+  - animal_detection_on_acoustic_recordings
+# - id: other_tools_label
+#   text: Other Tools
+#   type: label
+- sections:
+  - other_tools
+# - id: testing_tools_label
+#   text: Testing Tools
+#   type: label
+- sections:
+  - local_tools
+  - test_tools

files/galaxy-re.grid.cesnet.cz/pulsar.pub

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+ssh-ed25519 AAAAC3NzaC1lZDI1NTE5AAAAIPJ4pNj4pZBfm/hGSD04ZkuxtCbageNxwSD6EAZ+wyjQ galaxyelixir@galaxy-re.grid.cesnet.cz