cmeAnalysis (clathrin-mediated endocytosis analysis) is a MATLAB software package for the quantification of clathrin-coated pit dynamics from fluorescence time-lapse data. The functionalities provided include highly sensitive detection, tracking (based on u-track), master/slave detection for multi-channel data, intensity-based classification of coated structures, and lifetime analysis. Additionally, the package includes a graphical user interface for inspection of analysis results from individual movies. The download includes a user guide, and a test data set is available separately.
Release notes: includes improvements to graphical and console output.
For more information, please see Advances in Analysis of Low Signal-to-Noise Images Link Dynamin and AP2 to the Functions of an Endocytic Checkpoint, Developmental Cell, 2013, 26(3):279-291, written by François Aguet, Costin N. Antonescu, Marcel Mettlen, Sandra L. Schmid, Gaudenz Danuser.
“Disassembly asymmetry score classification” (DASC), is based on the new method developed in the article, DASC, a sensitive classifier for measuring discrete early stages in clathrin-mediated endocytosis, eLife, 2020, 9:e53686, written by Xinxin Wang, Zhiming Chen, Marcel Mettlen, Jungsik Noh, Sandra L Schmid, Gaudenz Danuser.
DASC is built upon and embedded in cmeAnalysis.
For more information, please read the User Guide.