Antibiotic screening

Background

2-aminothiazoles have been explored for their activity against Mtb in the past. Several of the bacteria responsible for actinomycetoma belong to the same class (Actinomycetia), and we wanted to see if 2-aminothiazoles that have been developed as anti-fungals might have activity against these bacteria as well.

The three bacteria being investigated are Actinomadura madurae (abbreviated to A. madurae or A.m.), Nocardia brasiliensis (abbreviated to N. brasiliensis or N.b.) and Streptomyces somaliensis (abbreviated to S. somaliensis or S.s.).

The high-low screening method used for antifungal screening has been applied to the compounds here. Approximately 60 compounds were screened at 100 and 25 uM. After this yielded around ~20 hits, the screening concentration was lowered to 2 uM for future compounds.

We are also interested in using resazurin dye to create a colorimetric screening process. This has been done before with A.m. but not the other bacteria - see this paper.