E coli K12 MG1655 Methylome

Instrument:  PacBio RS
Chemistry:  C2
Enzyme: C2

Summary

These data are intended for use with the Technical Note, Detecting DNA Base Modifications: SMRT® Analysis of Microbial Methylomes.

The examples include:

• The appropriate reference sequence FASTA file
• Five SMRT Cells of native E. coli data (Native Raw Reads HDF) with an analysis result comparing to the in silico control (Native Analysis)
• Six SMRT Cells of whole genome amplified data (WGA Raw Reads HDF) aligned to the appropriate reference
• An analysis of the native sequence data using the WGA as an amplified control (Native vs WGA Analysis)

Related Topics

Technical Note: Detecting DNA Base Modifications: SMRT® Analysis of Microbial Methylomes.

Download Datasets

Primary Analysis (Instrument Output) Data

• Native raw reads in HDF5 format 11.7 GB md5
• WGA raw reads in HDF5 format 12.3 GB md5

E coli K12 Reference Genome

• E coli K12 MG1655 Mutated FASTA 1.4 MB

Secondary Analysis Data

• Native Analysis 5.9 GB md5
• WGA Analysis 5.6 GB md5
• Native vs WGA Analysis 6.0 GB md5
• SMRT Analysis 1.3.3 Methylome Anlysis 5.5 GB

Tertiary Analysis Data

• The REBASE entry of predicted Modification sites for this organism is here.