Microbial Multiplexing Data Set: PacBio Sequel System, Chemistry v2.1, SMRT Link v5.1.0 Analysis

This is SMRT Sequencing data for a 10-plex multiplexed data set using the Barcoded Adapter 8A and Barcoded Adapter 8B kits. The pooled library contains a total of 42.4 Mb microbial genomes from different samples and has been sequenced on a single SMRT Cell. The libraries include duplicates of two PacBio control microbes and customer samples. Sequencing was performed on the Sequel System using Binding/Sequencing Chemistry v2.1 and analyzed with HGAP4 in SMRT Link v5.1.0. Assembled genomes have been circularized using the Circlator tool and polished through the Resequencing analysis on SMRT Link.

This data set accompanies Application Note: Microbial Multiplexing Workflow on the Sequel System, which details key considerations for experimental design and practices on the Sequel System.

Download Data: https://downloads.pacbcloud.com/public/dataset/microbial_multiplex_dataset_release_SMRT_Link_v5.1.0/

DATA SETS and supporting files (11 files):

1. MM_10Plex_43MB_SEQUENCING_DATA.tar.gz - Compressed gzipped file containing BAM file for original sequencing data and supporting .pbi and .xml files for importing into SMRT Link.
2. BC1002-Ecoli-control1-polished-assembly.fastq - Final polished genome assembly for E. coli multiplexed using barcoded adapter BC1002.
3. BC1004-Bsub-control1-polished-assembly.fastq - Final polished genome assembly for B. subtilis multiplexed using barcoded adapter BC1004.
4. BC1009-Ecoli-1-polished-assembly.fastq - Final polished genome assembly for E. coli multiplexed using barcoded adapter BC1009.
5. BC1012-Shigella-1-polished-assembly.fastq - Final polished genome assembly for _Shigella _multiplexed using barcoded adapter BC1012.
6. BC1014-Listeria-1-polished-assembly.fastq - Final polished genome assembly for _Listeria _multiplexed using barcoded adapter BC1014.
7. BC1015-Ecoli-control2-polished-assembly.fastq - Final polished genome assembly for E. coli multiplexed using barcoded adapter BC1015.
8. BC1016-Bsub-control2-polished-assembly.fastq - Final polished genome assembly for B. subtilis multiplexed using barcoded adapter BC1016.
9. BC1018-Ecoli-2-polished-assembly.fastq - Final polished genome assembly for E. coli multiplexed using barcoded adapter BC1018.
10. BC1020-Shigella-2-polished-assembly.fastq - Final polished genome assembly for _Shigella _multiplexed using barcoded adapter BC1020.
11. BC1022-Listeria-2-polished-assembly.fastq - Final polished genome assembly for Listeria multiplexed using barcoded adapter BC1022.

The following references on NCBI can be used for comparison with the respective microbes: CFSAN030807 Shigella sonnei, CFSAN001178 Listeria monocytogenes

DNA was extracted using the Circulomics Nanobind CBB Big DNA Kit for BC1009, BC1012, BC1014, BC1018, BC1020, BC1022. The Lucigen MasterPure Complete DNA and RNA Purification Kit was used to extract the PacBio Controls (BC1002, BC1004, BC1015, BC1016).