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Currently, HCV samples are sequenced and combined from two separate MiSeq samples: a whole-genome sample, and a MIDI sample. The new protocol for SARS-CoV-2 will combine about ten smaller amplicons with overlap. Try to trim the primers, as described in #552, then combine overlapping regions, and report on differences in the overlaps as a QC step. Also think through how this will work for the assembled version.
The text was updated successfully, but these errors were encountered:
In every SARS-CoV-2 run so far, we combined both PCR primer pools into a single "sample" prior to sequencing. Performance so far has been such that it probably won't be necessary to sequence the two pools separately and make micall combine the two sets of fastqs.
Currently, HCV samples are sequenced and combined from two separate MiSeq samples: a whole-genome sample, and a MIDI sample. The new protocol for SARS-CoV-2 will combine about ten smaller amplicons with overlap. Try to trim the primers, as described in #552, then combine overlapping regions, and report on differences in the overlaps as a QC step. Also think through how this will work for the assembled version.
The text was updated successfully, but these errors were encountered: