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Bump fgbio versions and swap to nftest #5624

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merged 18 commits into from
Jul 2, 2024
Merged

Bump fgbio versions and swap to nftest #5624

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d6cc2b3
Convert callduplex and callmolecular to nftest and update version
SPPearce May 17, 2024
01bd4c3
Convert zipperbams
SPPearce May 21, 2024
3fc5a08
Convert groupreadsbyumi
SPPearce May 21, 2024
5ec0a95
Convert sortbam and add stubs
SPPearce May 21, 2024
3d38cc5
Merge branch 'master' into fgbio_nftest
SPPearce May 21, 2024
0bfe648
Convert filterconsensusreads
SPPearce May 21, 2024
95ed61f
Update snapshot
SPPearce May 21, 2024
b1e1beb
Merge branch 'master' into fgbio_nftest
SPPearce May 21, 2024
7b18f7b
Merge branch 'master' into fgbio_nftest
SPPearce May 21, 2024
c3b5a1a
Update modules/nf-core/fgbio/callmolecularconsensusreads/main.nf
SPPearce May 21, 2024
49922ba
Add collision checks, swap test data paths
SPPearce May 21, 2024
abb2311
Merge branch 'master' into fgbio_nftest
SPPearce May 21, 2024
8d1dff3
Fix conda version and linting
SPPearce May 21, 2024
559fb11
Apply suggestions from code review
SPPearce May 22, 2024
b0589f1
Merge branch 'master' into fgbio_nftest
maxulysse Jun 17, 2024
ceb8df1
Update modules/nf-core/fgbio/callmolecularconsensusreads/meta.yml
SPPearce Jun 29, 2024
4a99079
Update duplex snap
SPPearce Jul 2, 2024
44458bc
Merge branch 'master' into fgbio_nftest
SPPearce Jul 2, 2024
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2 changes: 1 addition & 1 deletion modules/nf-core/fgbio/callduplexconsensusreads/environment.yml
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Original file line number Diff line number Diff line change
Expand Up @@ -4,4 +4,4 @@ channels:
- bioconda
- defaults
dependencies:
- bioconda::fgbio=2.0.2
- bioconda::fgbio=2.2.1
32 changes: 22 additions & 10 deletions modules/nf-core/fgbio/callduplexconsensusreads/main.nf
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Expand Up @@ -4,26 +4,24 @@ process FGBIO_CALLDUPLEXCONSENSUSREADS {

conda "${moduleDir}/environment.yml"
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/fgbio:2.0.2--hdfd78af_0' :
'biocontainers/fgbio:2.0.2--hdfd78af_0' }"
'https://depot.galaxyproject.org/singularity/fgbio:2.2.1--hdfd78af_0' :
'biocontainers/fgbio:2.2.1--hdfd78af_0' }"

input:
tuple val(meta), path(bam)
// please note:
// --min-reads is a required argument with no default
// --min-input-base-quality is a required argument with no default
// make sure they are specified via ext.args in your config
tuple val(meta), path(grouped_bam)
val min_reads
val min_baseq

output:
tuple val(meta), path("${prefix}.bam"), emit: bam
path "versions.yml" , emit: versions
path "versions.yml" , emit: versions

when:
task.ext.when == null || task.ext.when

script:
def args = task.ext.args ?: ''
prefix = task.ext.prefix ?: "${meta.id}_consensus"
prefix = task.ext.prefix ?: "${meta.id}_consensus_unmapped"
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def mem_gb = 8
if (!task.memory) {
Expand All @@ -43,8 +41,10 @@ process FGBIO_CALLDUPLEXCONSENSUSREADS {
--async-io=true \\
--compression=1 \\
CallDuplexConsensusReads \\
--input $bam \\
--input $grouped_bam \\
--output ${prefix}.bam \\
--min-reads ${min_reads} \\
--min-input-base-quality ${min_baseq} \\
--threads ${task.cpus} \\
$args

Expand All @@ -53,4 +53,16 @@ process FGBIO_CALLDUPLEXCONSENSUSREADS {
fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
END_VERSIONS
"""

stub:
prefix = task.ext.prefix ?: "${meta.id}_consensus"
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Suggested change
prefix = task.ext.prefix ?: "${meta.id}_consensus"
prefix = task.ext.prefix ?: "${meta.id} _consensus_unmapped"

"""
touch ${prefix}.bam

cat <<-END_VERSIONS > versions.yml
"${task.process}":
fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
END_VERSIONS
"""

}
13 changes: 9 additions & 4 deletions modules/nf-core/fgbio/callduplexconsensusreads/meta.yml
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Expand Up @@ -10,18 +10,23 @@ tools:
homepage: http://fulcrumgenomics.github.io/fgbio/
documentation: http://fulcrumgenomics.github.io/fgbio/tools/latest/CallDuplexConsensusReads.html
tool_dev_url: https://github.com/fulcrumgenomics/fgbio
licence: "['MIT']"
licence: ["MIT"]
input:
# Only when we have meta
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bam:
- grouped_bam:
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type: file
description: BAM/SAM file
description: BAM/SAM file, grouped by UMI
pattern: "*.{bam,sam}"
- min_reads:
type: integer
description: Minimum number of original reads to build each consensus read.
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- min_baseq:
type: integer
description: Minimum base quality score
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output:
- meta:
type: map
Expand Down
62 changes: 62 additions & 0 deletions modules/nf-core/fgbio/callduplexconsensusreads/tests/main.nf.test
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@@ -0,0 +1,62 @@
nextflow_process {

name "Test Process FGBIO_CALLDUPLEXCONSENSUSREADS"
script "../main.nf"
process "FGBIO_CALLDUPLEXCONSENSUSREADS"

tag "modules"
tag "modules_nfcore"
tag "fgbio"
tag "fgbio/callduplexconsensusreads"

test("homo_sapiens - bam") {

when {
process {
"""
input[0] = [
[ id:'test', single_end:false ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_duplex_umi_grouped_bam'], checkIfExists: true)
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]
input[1] = 3
input[2] = 20
"""
}
}

then {
assertAll(
{ assert process.success },
{ assert snapshot(process.out).match() }
)
}

}

test("homo_sapiens - stub") {

options "-stub"

when {
process {
"""
input[0] = [
[ id:'test', single_end:false ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_duplex_umi_grouped_bam'], checkIfExists: true)
]
input[1] = 3
input[2] = 20
"""
}
}

then {
assertAll(
{ assert process.success },
{ assert snapshot(process.out).match() }
)
}

}

}
72 changes: 72 additions & 0 deletions modules/nf-core/fgbio/callduplexconsensusreads/tests/main.nf.test.snap
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@@ -0,0 +1,72 @@
{
"homo_sapiens - stub": {
"content": [
{
"0": [
[
{
"id": "test",
"single_end": false
},
"test_consensus.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
]
],
"1": [
"versions.yml:md5,7277dba1bc055b578eb6d8d6af43b128"
],
"bam": [
[
{
"id": "test",
"single_end": false
},
"test_consensus.bam:md5,d41d8cd98f00b204e9800998ecf8427e"
]
],
"versions": [
"versions.yml:md5,7277dba1bc055b578eb6d8d6af43b128"
]
}
],
"meta": {
"nf-test": "0.8.4",
"nextflow": "23.10.1"
},
"timestamp": "2024-05-17T06:05:42.765492882"
},
"homo_sapiens - bam": {
"content": [
{
"0": [
[
{
"id": "test",
"single_end": false
},
"test_consensus_unmapped.bam:md5,4f0e87feb7601d06617c9f29d7aec352"
]
],
"1": [
"versions.yml:md5,7277dba1bc055b578eb6d8d6af43b128"
],
"bam": [
[
{
"id": "test",
"single_end": false
},
"test_consensus_unmapped.bam:md5,4f0e87feb7601d06617c9f29d7aec352"
]
],
"versions": [
"versions.yml:md5,7277dba1bc055b578eb6d8d6af43b128"
]
}
],
"meta": {
"nf-test": "0.8.4",
"nextflow": "23.10.1"
},
"timestamp": "2024-05-17T06:05:28.894178772"
}
}
2 changes: 2 additions & 0 deletions modules/nf-core/fgbio/callduplexconsensusreads/tests/tags.yml
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@@ -0,0 +1,2 @@
fgbio/callduplexconsensusreads:
- "modules/nf-core/fgbio/callduplexconsensusreads/**"
2 changes: 1 addition & 1 deletion modules/nf-core/fgbio/callmolecularconsensusreads/environment.yml
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Original file line number Diff line number Diff line change
Expand Up @@ -4,4 +4,4 @@ channels:
- bioconda
- defaults
dependencies:
- bioconda::fgbio=2.0.2
- bioconda::fgbio=2.2.1
39 changes: 32 additions & 7 deletions modules/nf-core/fgbio/callmolecularconsensusreads/main.nf
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Expand Up @@ -4,11 +4,13 @@ process FGBIO_CALLMOLECULARCONSENSUSREADS {

conda "${moduleDir}/environment.yml"
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/fgbio:2.0.2--hdfd78af_0' :
'biocontainers/fgbio:2.0.2--hdfd78af_0' }"
'https://depot.galaxyproject.org/singularity/fgbio:2.2.1--hdfd78af_0' :
'biocontainers/fgbio:2.2.1--hdfd78af_0' }"

input:
tuple val(meta), path(bam)
tuple val(meta), path(grouped_bam)
val min_reads
val min_baseq

output:
tuple val(meta), path("*.bam"), emit: bam
Expand All @@ -19,19 +21,42 @@ process FGBIO_CALLMOLECULARCONSENSUSREADS {

script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def prefix = task.ext.prefix ?: "${meta.id}_consensus_unmapped"
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Why did you add this custom suffix here?

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It needs some kind of name renaming to prevent it from colliding with the input file. Some of the subtools already done this, some don't. Although this one is now setting a double prefix in the output file, so that needs fixing.

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You can add a check for that (a lot of modules do this already) => https://nf-co.re/docs/guidelines/components/modules#command-file-output-naming

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Yes, it needs the checks whatever.
I think it is better to have a default prefix that doesn't require you to add one.

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I'm not one to argue with that, but I don't think diverting from the guidelines is a good idea, they're here for a reason. You can maybe bring this up on Slack?

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def mem_gb = 8
if (!task.memory) {
log.info '[fgbio CallMolecularConsensusReads] Available memory not known - defaulting to 8GB. Specify process memory requirements to change this.'
} else {
mem_gb = task.memory.giga
}
"""
fgbio \\
-Xmx${mem_gb}g \\
--tmp-dir=. \\
--async-io=true \\
--compression=1 \\
CallMolecularConsensusReads \\
--input $bam \\
--input $grouped_bam \\
--output ${prefix}.cons.unmapped.bam \\
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--min-reads ${min_reads} \\
--min-input-base-quality ${min_baseq} \\
--threads ${task.cpus} \\
$args \\
--output ${prefix}.bam
$args;

cat <<-END_VERSIONS > versions.yml
"${task.process}":
fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
END_VERSIONS
"""

stub:
prefix = task.ext.prefix ?: "${meta.id}_consensus_unmapped"
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"""
touch ${prefix}.bam

cat <<-END_VERSIONS > versions.yml
"${task.process}":
fgbio: \$( echo \$(fgbio --version 2>&1 | tr -d '[:cntrl:]' ) | sed -e 's/^.*Version: //;s/\\[.*\$//')
END_VERSIONS
"""

}
11 changes: 8 additions & 3 deletions modules/nf-core/fgbio/callmolecularconsensusreads/meta.yml
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Expand Up @@ -4,7 +4,6 @@ keywords:
- UMIs
- consensus sequence
- bam
- sam
tools:
- fgbio:
description: Tools for working with genomic and high throughput sequencing data.
Expand All @@ -17,11 +16,17 @@ input:
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false, collapse:false ]
- bam:
- grouped_bam:
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ditto, does the main.nf need to be updated?

type: file
description: |
The input SAM or BAM file.
The input SAM or BAM file, grouped by UMIs
pattern: "*.{bam,sam}"
- min_reads:
type: integer
description: Minimum number of original reads to build each consensus read.
- min_baseq:
type: integer
description: Minimum base quality score
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output:
- meta:
type: map
Expand Down
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