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ImRep across multiple samples
Serghei Mangul edited this page Feb 8, 2017
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This is relevant to release ROP v1.0.6. We are planning to incorporate this is the next ROP release
Prior to moving all assembled CDR3s into one place, we need to rename full_cdr3.txt to incorporate the sample name.
while read line; do mv ${line}/antibodyProfile/full_cdr3.txt full_cdr3_${line}.txt;done<samples.txt
Starting from ROP v1.0.7 you can run rop-imrep which will consider reads mapped to BCR and TCr loci together with unmapped reads.
To run across multiple samples, please use this command:
while read line ; do echo "~/code2/rop/rop-imrepGRCh38.sh /u/home/s/serghei/project/Catie/bam_sorted/HM-baseline1C_sort.bam $PWD/${line}/ \"\" ">run_${line}.sh;done<bam_sorted/samples.txt
Don’t let your unmapped reads go to waste
- Main
- About ROP Tutorial
- What is ROP?
- How ROP works?
- How to prepare unmapped reads
- How to customize tools used by ROP
- Unix Tutorial
- Get started
- Targeted analysis
- ROP analysis: one RNA-Seq sample
- How to run ROP for mouse
- ROP analysis via qsub
- ROP analysis of multiple samples via qsub array
- Immune profiling by ROP (ImReP)
- ImRep across multiple samples
- ROP input details
- ROP output details
- Source of every last read
- Additional options
- How to calculate immune diversity?
- How to run hyper editing pipeline?