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Decompress transcript fasta error #798

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rrdavis77 opened this issue Mar 29, 2022 · 1 comment
Closed

Decompress transcript fasta error #798

rrdavis77 opened this issue Mar 29, 2022 · 1 comment
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@rrdavis77
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Description of the bug

When providing a gz compressed transcript file with --transcript_fasta the pipeline errors with

[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GUNZIP_GTF -
Process `NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GUNZIP_TRANSCRIPT_FASTA` declares 1 input channel but 2 were specified

 -- Check script '/home/ryan/.nextflow/assets/nf-core/rnaseq/./workflows/../subworkflows/local/prepare_genome.nf' at line: 109 or see '.nextflow.log' file for more details

The error does not happen with providing a decompressed transcript fasta.

Command used and terminal output

nextflow run nf-core/rnaseq --input /home/ryan/NGS_Data/Wang_Hongbin_RNA-3-14-22/RNA_sample_list.csv --aligner star_salmon --pseudo_aligner salmon --gencode --outdir /home/ryan/NGS_Data/Carr-Ascher/RNAseq_analysis --fasta /home/ryan/Reference/Homo_sapiens/Ensembl/gencode_GRCH38/GRCh38.primary_assembly.genome.fa --gtf /home/ryan/Reference/Homo_sapiens/transcriptome/gencode.v39.annotation.gtf.gz --transcript_fasta /home/ryan/Reference/Homo_sapiens/transcriptome/gencode.v39.transcripts.fa.gz --save_reference --skip_stringtie --max_cpus 8 --max_memory 40GB -profile docker -c /home/ryan/NGS_Data/Wang_Hongbin_RNA-3-14-22/nextflow_rnaseq.config

WARN: =============================================================================
  '--transcript_fasta' parameter has been provided.
  Make sure transcript names in this file match those in the GFF/GTF file.

  Please see:
  https://github.com/nf-core/rnaseq/issues/753
===================================================================================
[-        ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GUNZIP_GTF -
Process `NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GUNZIP_TRANSCRIPT_FASTA` declares 1 input channel but 2 were specified

 -- Check script '/home/ryan/.nextflow/assets/nf-core/rnaseq/./workflows/../subworkflows/local/prepare_genome.nf' at line: 109 or see '.nextflow.log' file for more details

Relevant files

No response

System information

N E X T F L O W ~ version 21.10.3
Desktop
local executor
Docker container
Ubuntu 16.04
nfcore/rnaseq version 3.6

@rrdavis77 rrdavis77 added the bug Something isn't working label Mar 29, 2022
@drpatelh drpatelh added this to the 3.7 milestone Apr 26, 2022
@drpatelh drpatelh added the WIP Work in progress label Apr 26, 2022
drpatelh added a commit to drpatelh/nf-core-rnaseq that referenced this issue Apr 26, 2022
drpatelh added a commit that referenced this issue Apr 27, 2022
@drpatelh
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FIxed in #811

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