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--bam_csi_index error generated if --skip_alignment specified #802

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baptisteImbert opened this issue Apr 5, 2022 · 1 comment
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@baptisteImbert
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Description of the bug

Hi, the following bug was discussed on the Slack #rnaseq channel (4th March) and a workaround was identified.
When trying to use salmon pseudo-mapping quantification, an error asking to add "--bam_csi_index" because of long chromosomes will show up, despite the parameter being already mentionned.
This issue does not appear on v3.5.

Command used and terminal output

nextflow run nf-core/rnaseq -profile docker --input samplesheet.csv --fasta psat_genome.fa --gtf psat_annotation.gtf --pseudo_aligner salmon --skip_alignment --bam_csi_index --outdir results_rnaseq/henriet2019_sample


  Contig longer than 512000000bp found in reference genome!

  chr5LG3: 579269071

  Provide the '--bam_csi_index' parameter to use a CSI instead of BAI index.

  Please see:
  https://github.com/nf-core/rnaseq/issues/744

Relevant files

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System information

Nextflow version 21.10.6
nf-core/rnaseq 3.6

@baptisteImbert baptisteImbert added the bug Something isn't working label Apr 5, 2022
@drpatelh drpatelh added this to the 3.7 milestone Apr 26, 2022
@drpatelh drpatelh changed the title salmon --skip_alignment raises --bam_csi_index despite being given as an argument --bam_csi_index error generated if --skip_alignment specified Apr 26, 2022
@drpatelh drpatelh added the WIP Work in progress label Apr 26, 2022
drpatelh added a commit to drpatelh/nf-core-rnaseq that referenced this issue Apr 26, 2022
drpatelh added a commit that referenced this issue Apr 27, 2022
@drpatelh
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Fixed in #811

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