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Following this discussion on Slack, after running joint calling and VQSR, it would be helpful to produce a final VCF file with filtered variants, i.e. those that have PASS on their filter column, and not . or anything else. Something like the following bcftools command:
The current behavior is to have a mix of both calibrated and uncalibrated variants in joint_germline_recalibrated.vcf.gz, which means that there are two records for each variant.
The text was updated successfully, but these errors were encountered:
Description of feature
Following this discussion on Slack, after running joint calling and VQSR, it would be helpful to produce a final VCF file with filtered variants, i.e. those that have
PASS
on their filter column, and not.
or anything else. Something like the following bcftools command:The current behavior is to have a mix of both calibrated and uncalibrated variants in
joint_germline_recalibrated.vcf.gz
, which means that there are two records for each variant.The text was updated successfully, but these errors were encountered: