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SB Shuffle sequences

Steve Bond edited this page Oct 18, 2015 · 6 revisions

--shuffle_seqs, -ss

Description

Randomly rearrange the residues in each record (the total composition remains unchanged). This may be useful if performing statistical tests where overall composition may influence the significance of your result.

Example

Input file: Mle-Panxα2.fa

>Mle-Panxα2 - ML25998a.
MVLDLISGSLNGFLKIKSVSIDDQWDQINRTYLVMFCILSGTIMTFKQNLGSIIHCISDA
RGDDSSFADAHATFVQDYCAAQGLYTLKEVYDKSWPDEIPYPGILQMKTIGCFPGRQFKN
GTPIQCPDEKDLKPFTTVYHVWYMFVPFYFCAVGIAFYFPYTVFRHLSGIYDIKPMLNSL
ALDIGAYTEEDISRRIDNVSRWLYIKLDPYMNNMLPYTQIVHKHSIFYTVMLVKVMYLAT
SVSIFYATHRIFDQGNFALYGYDVLMSIPQETSYKVMDTIFPKMVGCEINMWGRTGEQSE
SLLCVLPQNIGNQYFFLIFWFLLILTILSNCISVIVTIFRFIFVSGSYKRFLATSLLNHE
ERYKLVFTHVGTTGRYILLLCADHSNPKIFEDLLEIVCSLLIANYHKRKRSRDKGHSRAE
GVGTKGRHGLSFVDSTV

Usage

$: sb Mle-Panxα2.fa -ss

Output

>Mle-Panxα2 cDNA - ML25998a.
TLSIFWGVLWYYAFSPTKPEKQTISWQVKVLLKDVQVIALIYVVACCHEDNVFYSPLPFA
LHSEAVHQRFTVGGVILVSLMSLARISVEDSHTNIGDYYFNYITGCTAISSLGGGHYLPG
SILFTMMSGVGLNILYEIKDMLEKDSFRKFLILAKTIIRTSILGKTLHKFGA*SFCRGRF
FVPRQCAPIYDRTKDILPRMIGVFDEYFCSLFITNSMIKRIMTSLVQMIGTLGNFNMYIL
WHITRSLGGHDQKQLQLSRYKHTVVSQTDLYPVQFLCYQGFLIERSSTVIYKYMYEQKCL
LSDSVAPADWALEIATYSLSRTDFKKLEVAPSRIANYHCFDGFVCGPPVDVYPYMGTGFD
DYMNTNLDMGFHFVIIPLGNLVLAEKVSPIWYINNRKGDFIIDYKNETNESDSHIIFYQD
DIIMTHLKFNRFTRLDCF

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